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KMID : 0360220150560010099
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Journal of the Korean Ophthalmological Society
2015 Volume.56 No. 1 p.99 ~ p.103
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Transcriptional Analysis of Nod-Like Receptors in a Mouse Model of Experimental Autoimmune Uveitis
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Kim Hyeong-Min
Kim Tae-Wan
Jeong Hyun-Jeong
Ryu Jin-Suk
Kim Mee-Kum
Wee Won-Ryang
Oh Joo-Youn
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Abstract
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Purpose: To evaluate the transcription pattern of Nod-like receptors (NLRs), the intracellular sensors, to detect danger signals in murine eyes with experimental autoimmune uveitis (EAU).
Methods: EAU was induced in B6 (C57BL/6) mice by subcutaneous injection of human interphotoreceptor retinoid binding protein and intraperitoneal injection of pertussis toxin. At 1, 2, and 3 weeks post-immunization, the eyeballs were extracted and subjected to histological and molecular assays using real-time reverse transcription polymerase chain reaction.
Results: The levels of nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain 1 (NLRP1), NLRP3, nucleotide- binding oligomerization domain-containing protein 1 (NOD1), and NOD2 transcripts were increased at 2 weeks and gradually reduced thereafter. Notably, NLRP3 showed the highest expression in the eyes with EAU. Similarly, the transcript level of pro-inflammatory cytokine, interleukin-1β, increased and reached a peak at 2 weeks post-immunization. The retinal structure was severely damaged by inflammation at 3 weeks post-immunization.
Conclusions: Among NLRs, NLRP3 may induce inflammation in eyes after EAU immunization.
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KEYWORD
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Experimental autoimmune uveitis, NLRP3, Nod-like receptors
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